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Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance

Received: 26 October 2022     Accepted: 10 November 2022     Published: 23 November 2022
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Abstract

Phosphate-solubilizing microorganisms (PSMs) are able to dissolve insoluble phosphorus in the soil. They play an important role in promoting soil nutrient cycling, plant growth, and dissolving insoluble phosphorus in the soil, and are therefore important microbial resources in biological fertilizers. In this study, the PSMs in rice rhizosphere soil were isolated and purified. Twenty-one phosphate-solubilizing strains were screened, including 6 bacteria and 15 fungi. Morphological analysis and molecular identification of the phosphate-solubilizing bacteria were conducted to determine the species. According to 16S rRNA molecular identification, the phosphate-solubilizing bacteria belonged to Pseudomonas aeruginosa, Bacillus albus, Bacillus subtilis, and Rhizobium sp. The phosphate-solubilizing ability of the PSMs was determined using a plate containing tricalcium phosphate (TCP). The results showed that the phosphate-solubilizing index of most of the PSMs increased first and then decreased. The phosphate-solubilizing ability of the PSMs toward the inorganic phosphate TCP, iron phosphate, aluminum phosphate, and organophosphorus of lecithin was measured using the liquid method. The results showed that the phosphate-solubilizing ability of the PSMs was 0.39mg/L–494.13mg/L, and the phosphate-solubilizing ability of TCP was the best. Among them, 13 strains had phosphate-solubilizing ability above 100 mg/L. In this study, Twenty-one strains of highly efficient phosphate-solubilizing bacteria were isolated, providing strain resources and a theoretical reference for the development of phosphate-solubilizing biological fertilizer.

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 4)
DOI 10.11648/j.ijmb.20220704.13
Page(s) 169-176
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Phosphate-Solubilizing Microorganisms, Rice Rhizosphere Soil, Phosphate-Solubilizing Ability

References
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    Huanhuan Jiang, iaqin Li, Honghuo He, Minjia Zhang, Liang Yunyan, et al. (2022). Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. International Journal of Microbiology and Biotechnology, 7(4), 169-176. https://doi.org/10.11648/j.ijmb.20220704.13

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    ACS Style

    Huanhuan Jiang; iaqin Li; Honghuo He; Minjia Zhang; Liang Yunyan, et al. Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. Int. J. Microbiol. Biotechnol. 2022, 7(4), 169-176. doi: 10.11648/j.ijmb.20220704.13

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    AMA Style

    Huanhuan Jiang, iaqin Li, Honghuo He, Minjia Zhang, Liang Yunyan, et al. Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. Int J Microbiol Biotechnol. 2022;7(4):169-176. doi: 10.11648/j.ijmb.20220704.13

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  • @article{10.11648/j.ijmb.20220704.13,
      author = {Huanhuan Jiang and iaqin Li and Honghuo He and Minjia Zhang and Liang Yunyan and Fan Jingyu and Wang Jingyi and Mo Cuiting and Sainan Li and Na Lv Yi},
      title = {Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {7},
      number = {4},
      pages = {169-176},
      doi = {10.11648/j.ijmb.20220704.13},
      url = {https://doi.org/10.11648/j.ijmb.20220704.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.13},
      abstract = {Phosphate-solubilizing microorganisms (PSMs) are able to dissolve insoluble phosphorus in the soil. They play an important role in promoting soil nutrient cycling, plant growth, and dissolving insoluble phosphorus in the soil, and are therefore important microbial resources in biological fertilizers. In this study, the PSMs in rice rhizosphere soil were isolated and purified. Twenty-one phosphate-solubilizing strains were screened, including 6 bacteria and 15 fungi. Morphological analysis and molecular identification of the phosphate-solubilizing bacteria were conducted to determine the species. According to 16S rRNA molecular identification, the phosphate-solubilizing bacteria belonged to Pseudomonas aeruginosa, Bacillus albus, Bacillus subtilis, and Rhizobium sp. The phosphate-solubilizing ability of the PSMs was determined using a plate containing tricalcium phosphate (TCP). The results showed that the phosphate-solubilizing index of most of the PSMs increased first and then decreased. The phosphate-solubilizing ability of the PSMs toward the inorganic phosphate TCP, iron phosphate, aluminum phosphate, and organophosphorus of lecithin was measured using the liquid method. The results showed that the phosphate-solubilizing ability of the PSMs was 0.39mg/L–494.13mg/L, and the phosphate-solubilizing ability of TCP was the best. Among them, 13 strains had phosphate-solubilizing ability above 100 mg/L. In this study, Twenty-one strains of highly efficient phosphate-solubilizing bacteria were isolated, providing strain resources and a theoretical reference for the development of phosphate-solubilizing biological fertilizer.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance
    AU  - Huanhuan Jiang
    AU  - iaqin Li
    AU  - Honghuo He
    AU  - Minjia Zhang
    AU  - Liang Yunyan
    AU  - Fan Jingyu
    AU  - Wang Jingyi
    AU  - Mo Cuiting
    AU  - Sainan Li
    AU  - Na Lv Yi
    Y1  - 2022/11/23
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijmb.20220704.13
    DO  - 10.11648/j.ijmb.20220704.13
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 169
    EP  - 176
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20220704.13
    AB  - Phosphate-solubilizing microorganisms (PSMs) are able to dissolve insoluble phosphorus in the soil. They play an important role in promoting soil nutrient cycling, plant growth, and dissolving insoluble phosphorus in the soil, and are therefore important microbial resources in biological fertilizers. In this study, the PSMs in rice rhizosphere soil were isolated and purified. Twenty-one phosphate-solubilizing strains were screened, including 6 bacteria and 15 fungi. Morphological analysis and molecular identification of the phosphate-solubilizing bacteria were conducted to determine the species. According to 16S rRNA molecular identification, the phosphate-solubilizing bacteria belonged to Pseudomonas aeruginosa, Bacillus albus, Bacillus subtilis, and Rhizobium sp. The phosphate-solubilizing ability of the PSMs was determined using a plate containing tricalcium phosphate (TCP). The results showed that the phosphate-solubilizing index of most of the PSMs increased first and then decreased. The phosphate-solubilizing ability of the PSMs toward the inorganic phosphate TCP, iron phosphate, aluminum phosphate, and organophosphorus of lecithin was measured using the liquid method. The results showed that the phosphate-solubilizing ability of the PSMs was 0.39mg/L–494.13mg/L, and the phosphate-solubilizing ability of TCP was the best. Among them, 13 strains had phosphate-solubilizing ability above 100 mg/L. In this study, Twenty-one strains of highly efficient phosphate-solubilizing bacteria were isolated, providing strain resources and a theoretical reference for the development of phosphate-solubilizing biological fertilizer.
    VL  - 7
    IS  - 4
    ER  - 

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Author Information
  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

  • School of Life Sciences, Zhaoqing University, Zhaoqing, China

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