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In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins

Received: 12 August 2022     Accepted: 7 September 2022     Published: 13 January 2023
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Abstract

Present study describes isolation of potential probiotic lactic acid bacteria from chicken crop, human feces, buttermilk and chilly. The isolated Lactobacillus strains survive, tolerate and grow in MRS medium spiked with bile, salt and having acidic pH. The Lactobacillus isolates possess several probiotic properties, viz. (i) ability to bind gastrointestinal mucosa, up to ≥80% cells adhered mucin, (ii) 50% cells retained viability during oro-gastro-intestinal transit, (iii) all the isolates exhibited broad anti-microbial spectrum against food spoilage and gastro-intestinal pathogens, Limosilactobacillus fermentum SBM showed maximum inhibition, (iv) ability to produce enzymatic activities like L-asparaginase, β-galactosidase and bile salt hydrolase (BSH) activities, Limosilactobacillus fermentum SBM showed maximum L-asparaginase activity (2.567 U/ml), and Lactiplantibacillus pentosus GCHI showed maximum β-galactosidase activity (296±0.1 Miller’s Unit), (v) Lactiplantibacillus pentosus GCHI aggregated up to ≥92% after 24 h, and (vi) the Lactobacillus isolates were susceptible towards nucleic acid synthesis inhibitors and cell wall synthesis inhibitor antibiotics. These Lactobacillus strains do not possess haemolytic, mucin degrading and DNase activities indicating their safety. Further characterization of these strains indicated potential probiotic properties and their suitability in food formulations as probiotics. The study presents an interesting illustration of mining of potential probiotic strains from nature exhibiting health benefits for human being and animals.

Published in International Journal of Microbiology and Biotechnology (Volume 8, Issue 1)
DOI 10.11648/j.ijmb.20230801.11
Page(s) 1-9
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Anti-Microbial Activity, BSH, Lactobacillus Strains, L-asparaginase, Probiotics, Safety Evaluation

References
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    Nilofar Yunus Bhatti, Arati Savji Chavda, Bharatkumar Rajiv Manuel Vyas. (2023). In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. International Journal of Microbiology and Biotechnology, 8(1), 1-9. https://doi.org/10.11648/j.ijmb.20230801.11

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    Nilofar Yunus Bhatti; Arati Savji Chavda; Bharatkumar Rajiv Manuel Vyas. In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. Int. J. Microbiol. Biotechnol. 2023, 8(1), 1-9. doi: 10.11648/j.ijmb.20230801.11

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    AMA Style

    Nilofar Yunus Bhatti, Arati Savji Chavda, Bharatkumar Rajiv Manuel Vyas. In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins. Int J Microbiol Biotechnol. 2023;8(1):1-9. doi: 10.11648/j.ijmb.20230801.11

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  • @article{10.11648/j.ijmb.20230801.11,
      author = {Nilofar Yunus Bhatti and Arati Savji Chavda and Bharatkumar Rajiv Manuel Vyas},
      title = {In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {8},
      number = {1},
      pages = {1-9},
      doi = {10.11648/j.ijmb.20230801.11},
      url = {https://doi.org/10.11648/j.ijmb.20230801.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230801.11},
      abstract = {Present study describes isolation of potential probiotic lactic acid bacteria from chicken crop, human feces, buttermilk and chilly. The isolated Lactobacillus strains survive, tolerate and grow in MRS medium spiked with bile, salt and having acidic pH. The Lactobacillus isolates possess several probiotic properties, viz. (i) ability to bind gastrointestinal mucosa, up to ≥80% cells adhered mucin, (ii) 50% cells retained viability during oro-gastro-intestinal transit, (iii) all the isolates exhibited broad anti-microbial spectrum against food spoilage and gastro-intestinal pathogens, Limosilactobacillus fermentum SBM showed maximum inhibition, (iv) ability to produce enzymatic activities like L-asparaginase, β-galactosidase and bile salt hydrolase (BSH) activities, Limosilactobacillus fermentum SBM showed maximum L-asparaginase activity (2.567 U/ml), and Lactiplantibacillus pentosus GCHI showed maximum β-galactosidase activity (296±0.1 Miller’s Unit), (v) Lactiplantibacillus pentosus GCHI aggregated up to ≥92% after 24 h, and (vi) the Lactobacillus isolates were susceptible towards nucleic acid synthesis inhibitors and cell wall synthesis inhibitor antibiotics. These Lactobacillus strains do not possess haemolytic, mucin degrading and DNase activities indicating their safety. Further characterization of these strains indicated potential probiotic properties and their suitability in food formulations as probiotics. The study presents an interesting illustration of mining of potential probiotic strains from nature exhibiting health benefits for human being and animals.},
     year = {2023}
    }
    

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    T1  - In Vitro Evaluation of Putative Probiotic Candidates Isolated from Various Origins
    AU  - Nilofar Yunus Bhatti
    AU  - Arati Savji Chavda
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    AB  - Present study describes isolation of potential probiotic lactic acid bacteria from chicken crop, human feces, buttermilk and chilly. The isolated Lactobacillus strains survive, tolerate and grow in MRS medium spiked with bile, salt and having acidic pH. The Lactobacillus isolates possess several probiotic properties, viz. (i) ability to bind gastrointestinal mucosa, up to ≥80% cells adhered mucin, (ii) 50% cells retained viability during oro-gastro-intestinal transit, (iii) all the isolates exhibited broad anti-microbial spectrum against food spoilage and gastro-intestinal pathogens, Limosilactobacillus fermentum SBM showed maximum inhibition, (iv) ability to produce enzymatic activities like L-asparaginase, β-galactosidase and bile salt hydrolase (BSH) activities, Limosilactobacillus fermentum SBM showed maximum L-asparaginase activity (2.567 U/ml), and Lactiplantibacillus pentosus GCHI showed maximum β-galactosidase activity (296±0.1 Miller’s Unit), (v) Lactiplantibacillus pentosus GCHI aggregated up to ≥92% after 24 h, and (vi) the Lactobacillus isolates were susceptible towards nucleic acid synthesis inhibitors and cell wall synthesis inhibitor antibiotics. These Lactobacillus strains do not possess haemolytic, mucin degrading and DNase activities indicating their safety. Further characterization of these strains indicated potential probiotic properties and their suitability in food formulations as probiotics. The study presents an interesting illustration of mining of potential probiotic strains from nature exhibiting health benefits for human being and animals.
    VL  - 8
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Author Information
  • Department of Biosciences, Saurashtra University, Rajkot, India

  • Department of Biosciences, Saurashtra University, Rajkot, India

  • Department of Biosciences, Saurashtra University, Rajkot, India

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